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Co-localization analysis of fluorescence microscopy images

A few months ago, I wasn’t sure what to expect when looking at fluorescence microscopy images in published papers. I looked at the accompanying graph to understand the data or the point the authors were trying to make. Often, the graph represents one or more measures of the so-called co-localization, but I couldn’t figure out how to interpret them. It turned out; reading the images is simple. Cells are simultaneously stained by two dyes (say, red and green) for two different proteins....

Checklist Recipe - How we created a template to standardize species data

Imagine you are a fish ecologist who compiled a list of fish species for your country. 🐟 Your list could be useful to others, so you publish it as a supplementary file to an article or in a research repository. That is fantastic, but it might be difficult for others to discover your list or combine it with other lists of species. Luckily there’s a better way to publish species lists: as a standardized checklist that can be harvested and processed by the Global Biodiversity Information Facility (GBIF)....

The Antarctic/Southern Ocean rOpenSci community

Antarctic/Southern Ocean science and rOpenSci Collaboration and reproducibility are fundamental to Antarctic and Southern Ocean science, and the value of data to Antarctic science has long been promoted. The Antarctic Treaty (which came into force in 1961) included the provision that scientific observations and results from Antarctica should be openly shared. The high cost and difficulty of acquisition means that data tend to be re-used for different studies once collected....

Sharing the Recipe for rOpenSci's Unconf Ice Breaker

While many people groan at the thought of participating in a group ice breaker activity, we’ve gotten consistent feedback from people who have been to recent rOpenSci unconferences. Best ice breaker ever! We’ve had lots of requests for a detailed description of how we do it. This post shares our recipe, including a script you can adapt, a reflection on its success, examples of how others have used it, and some tips to remember....

Community Call - Working with images in R

rOpenSci’s software engineer / postdoc Jeroen Ooms will explain what images are, under the hood, and showcase several rOpenSci packages that form a modern toolkit for working with images in R, including opencv, av, tesseract, magick and pdftools. 🕘 Thursday, November 15, 2018, 10-11AM PST; 7-8PM CET (find your timezone) ☎️ Find all details for joining the call on our Community Calls page. Everyone is welcome. No RSVP needed. Agenda Welcome (Stefanie Butland, rOpenSci Community Manager, 5 min) Working with images in R (Jeroen Ooms, 35 min) Q & A (20 min) Abstract Images in various forms are used for numerous applications across scientific disciplines....

Working together to push science forward

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